Anti-wrinkle treatments are performed using an injection of botulinum toxin - also known by its brand name BOTOX. It is a naturally made substance that is found in the soil, but is purified for cosmetic and clinical use. It has been used for over 20 years with no adverse effects.
When precise amounts are injected into the small muscles being treated, it causes the muscles to relax which prevents muscle contraction and achieves the desired cosmetic effect of leaving the skin wrinkle free. The muscles are still able to function as normal after the treatment, and normal facial expressions are unaffected.